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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
27
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pubmed:dateCreated |
1988-10-19
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pubmed:abstractText |
Intracellular free Ca2+ concentrations were monitored in vascular smooth muscle cells (VSMC) using the Ca2+-sensitive dye fura II. Superfusion of VSMC with platelet-activating factor (S-PAF; 1-100 nM) increased cytosolic Ca2+ in a dose-dependent manner. The response was transient and returned to base line even though the agonist was still present. A second, higher dose of PAF did not elicit a response. The inactive optical isomer, R-PAF, was ineffective suggesting that the S-PAF response is specific and receptor-mediated. Pretreatment of VSMC with PAF attenuated angiotensin II-stimulated Ca2+ mobilization but not vasopressin-stimulated Ca2+ mobilization. Treatment of VSMC with PAF (10 nM) stimulated inositol trisphosphate and inositol tetrakisphosphate formation above control by 260 +/- 15% and 195 +/- 11%, respectively. Diacylglycerol levels also rose during PAF stimulation and remained increased over 15 min. Pretreatment of VSMCs with phorbol-12,13-myristate acetate (10 nM) for 30 min abolished both the PAF- and angiotensin II-induced increases in cytosolic Ca2+, but not the vasopressin-induced increase. Pretreatment of VSMC with dioctanoylglycerol (10 microM) abolished the S-PAF-, angiotensin II-, and vasopressin-induced elevation in cytosolic Ca2+. We propose that this desensitization is possibly mediated by diacylglycerol formed in response to PAF.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Diglycerides,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet Activating Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Vasopressins
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
263
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13791-6
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3262105-Angiotensin II,
pubmed-meshheading:3262105-Animals,
pubmed-meshheading:3262105-Aorta,
pubmed-meshheading:3262105-Benzofurans,
pubmed-meshheading:3262105-Calcium,
pubmed-meshheading:3262105-Cells, Cultured,
pubmed-meshheading:3262105-Cytosol,
pubmed-meshheading:3262105-Diglycerides,
pubmed-meshheading:3262105-Fluorescent Dyes,
pubmed-meshheading:3262105-Fura-2,
pubmed-meshheading:3262105-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:3262105-Inositol Phosphates,
pubmed-meshheading:3262105-Male,
pubmed-meshheading:3262105-Muscle, Smooth, Vascular,
pubmed-meshheading:3262105-Platelet Activating Factor,
pubmed-meshheading:3262105-Rats,
pubmed-meshheading:3262105-Rats, Inbred Strains,
pubmed-meshheading:3262105-Spectrometry, Fluorescence,
pubmed-meshheading:3262105-Tetradecanoylphorbol Acetate,
pubmed-meshheading:3262105-Vasopressins
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pubmed:year |
1988
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pubmed:articleTitle |
Platelet-activating factor-induced homologous and heterologous desensitization in cultured vascular smooth muscle cells.
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pubmed:affiliation |
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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