|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1988-9-27
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
A subpopulation of the CD3+ peripheral T lymphocytes express the TCR-gamma/delta complex. Three distinct TCR-gamma forms that differ in size and in the ability to form a disulfide bridge with the TCR-delta subunit have been described. In this study we analyze the structural difference between the non-disulfide-linked 55-kD and 40-kD TCR-gamma chains. The 40-kD TCR-gamma form contains a smaller polypeptide backbone and carries less carbohydrate compared with the 55-kD TCR-gamma form. A cDNA clone corresponding to the 40-kD TCR-gamma subunit lacks one copy of the second exon of the constant region that is present in the other TCR-gamma subunit. This exon copy encodes part of the connector region that is located between the constant domain and the membrane spanning region. We show that the number of potential N-linked glycan attachment sites are the same for the two TCR-gamma forms. Since these attachment sites are located in the connector region we conclude that the connector region influences the amount of N-linked carbohydrates added to the core TCR-gamma polypeptide, probably by affecting the conformation of the protein. In contrast to the TCR-beta constant region usage, the TCR-gamma constant regions are unequally expressed. Virtually exclusive usage of disulfide-linked complexes were found in some individuals, while both the disulfide-linked and the 40-kD, non-disulfide-linked TCR-gamma forms were detected in other subjects. The ability to distinguish these TCR-gamma/delta forms now makes it possible to study the mechanisms that govern their selection and to determine if they correspond to functionally distinct isotypes.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-1172191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2410507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2435832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2439914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2820713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2825032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2879283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2938743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2951442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2961997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-2993909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3003579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3079918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3092224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3102966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3102967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3108883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3112943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3156277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3259726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3458221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3486244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3486721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3487737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3492555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3498213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3499667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3605611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3672118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3755221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3787252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3866244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-3917858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-4063349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-4204552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-4563441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-6165796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-6330561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-6432935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-6437277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3261778-6821395
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0022-1007
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
168
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
N
|
|
pubmed:pagination |
761-76
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
1988
|
|
pubmed:articleTitle |
Characterization of a third form of the human T cell receptor gamma/delta.
|
|
pubmed:affiliation |
Division of Tumor Virology, Dana-Faber Cancer Institute, Boston, Massachusetts.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
