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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-8-24
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pubmed:abstractText |
Glomerular mesangial cell (MC)--derived IL-1 may be an important factor in the development of the hypercellularity and sclerosis characteristic of many forms of glomerulonephritis. To define the regulation of IL-1 synthesis by human MC, Northern blot analyses were performed using specific probes for monocytic IL-1 alpha and beta mRNA. Proliferating MC expressed mRNA for both IL-1 alpha and beta, whereas nonproliferating MC contained no detectable IL-1 mRNA. Synchronized MC expressed IL-1 alpha and beta mRNA within 2 h of stimulation with serum. This serum effect could be reproduced with platelet-derived growth factor and epidermal growth factor. Immune precipitations of 35S-methionine-labeled cells indicate that the mesangial IL-1 is synthesized as a 33-kD precursor protein with a pI of 7.2. Extracellular mesangial IL-1 has a pI of 7.0 and molecular weight of 17 kD, consistent with its identification as IL-1 beta. Cellular proliferation in glomerular disease may be driven in part by peptide growth factor-mediated induction of mesangial IL-1 gene expression and protein synthesis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-2420874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-2431094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-2578046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-2989698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3021848,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3314532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3485151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3486219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3491091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3492551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3494069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3526909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3535527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3624483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3871212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3871836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3875682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3892153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-3894792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-532963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6209582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6300879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6311192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6327124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6365933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6405073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6601138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6603475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6605992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6607284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6700722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6801132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-6982921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-7144055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3260603-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
115-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3260603-Cell Cycle,
pubmed-meshheading:3260603-Cells, Cultured,
pubmed-meshheading:3260603-Extracellular Matrix,
pubmed-meshheading:3260603-Gene Expression Regulation,
pubmed-meshheading:3260603-Glomerular Mesangium,
pubmed-meshheading:3260603-Growth Substances,
pubmed-meshheading:3260603-Humans,
pubmed-meshheading:3260603-Interleukin-1,
pubmed-meshheading:3260603-Interphase,
pubmed-meshheading:3260603-Kinetics,
pubmed-meshheading:3260603-RNA, Messenger
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pubmed:year |
1988
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pubmed:articleTitle |
Cell cycle-dependent interleukin 1 gene expression by cultured glomerular mesangial cells.
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pubmed:affiliation |
Medical Service, Veterans Administration Medical Center, San Francisco, CA 94121.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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