|
rdf:type |
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lifeskim:mentions |
|
|
pubmed:issue |
1
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pubmed:dateCreated |
1988-7-18
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|
pubmed:abstractText |
Utilizing an improved method for colony hybridization developed by Haas & Fleming, biotin and 32P-labelled TEM-1 probes were compared for sensitivity and specificity in identifying the type of beta-lactamase made by over 100 clinical bacterial isolates. The new procedure was more reliable than a standard one, but still gave more than 20% false positive and false negative reactions.
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pubmed:grant |
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Mar
|
|
pubmed:issn |
0890-8508
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pubmed:author |
|
|
pubmed:issnType |
Print
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pubmed:volume |
2
|
|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
83-5
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|
pubmed:dateRevised |
2008-8-22
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pubmed:meshHeading |
pubmed-meshheading:3260006-Bacteria,
pubmed-meshheading:3260006-Bacterial Proteins,
pubmed-meshheading:3260006-Biotin,
pubmed-meshheading:3260006-DNA, Recombinant,
pubmed-meshheading:3260006-Drug Resistance, Microbial,
pubmed-meshheading:3260006-Isoelectric Focusing,
pubmed-meshheading:3260006-Nucleic Acid Hybridization,
pubmed-meshheading:3260006-Predictive Value of Tests,
pubmed-meshheading:3260006-beta-Lactamases
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|
pubmed:year |
1988
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|
pubmed:articleTitle |
Reliability of biotinylated DNA probes in colony hybridization: evaluation of an improved colony lysis method for detection of TEM-1 beta-lactamase.
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|
pubmed:affiliation |
Massachusetts General Hospital, Boston 02114.
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|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
