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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1988-6-9
|
| pubmed:databankReference | |
| pubmed:abstractText |
We have isolated the Ly-3 gene by chromosomal walking from the previously cloned Ly-2 gene. These genes are shown to be 36 kb apart and in the same transcriptional orientation. Transfection of the Ly-3 gene into mouse L cells results in cell surface expression of Ly-3 protein only in the presence of Ly-2 (or its human homolog, CD8), although Ly-2 surface expression is not similarly dependent on Ly-3. cDNA clones encoding Ly-3 have been isolated and sequenced and show little sequence similarity to Ly-2, whereas both Ly-2 and Ly-3 are homologous to Ig variable regions. One cDNA clone encodes a form of Ly-3 lacking a transmembrane region. Although two alleles of Ly-3 have been previously defined serologically, Northern blot analyses of Ly-3 mRNA from a series of inbred mouse strains show unusual polymorphisms in the lengths of Ly-3 mRNA species defining at least three allelic variants of this gene.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
140
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3646-53
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:3258885-Alleles,
pubmed-meshheading:3258885-Amino Acid Sequence,
pubmed-meshheading:3258885-Animals,
pubmed-meshheading:3258885-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:3258885-Antigens, Ly,
pubmed-meshheading:3258885-Base Sequence,
pubmed-meshheading:3258885-Cell Membrane,
pubmed-meshheading:3258885-Cloning, Molecular,
pubmed-meshheading:3258885-Genes, MHC Class I,
pubmed-meshheading:3258885-Genetic Linkage,
pubmed-meshheading:3258885-Humans,
pubmed-meshheading:3258885-Mice,
pubmed-meshheading:3258885-Mice, Inbred BALB C,
pubmed-meshheading:3258885-Mice, Inbred C57BL,
pubmed-meshheading:3258885-Mice, Inbred DBA,
pubmed-meshheading:3258885-Molecular Sequence Data,
pubmed-meshheading:3258885-Nucleic Acid Conformation,
pubmed-meshheading:3258885-RNA, Messenger,
pubmed-meshheading:3258885-Rats
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Molecular linkage of the Ly-3 and Ly-2 genes. Requirement of Ly-2 for Ly-3 surface expression.
|
| pubmed:affiliation |
Department of Medicine, Stanford University Medical Center, CA 94305.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|