Linked Life Data

3258638

Source:http://linkedlifedata.com/resource/pubmed/id/3258638

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1988-5-26
pubmed:abstractText
Three murine lymphomas of H-2a origin were investigated. Each of these lymphomas was derived in B10.H-2aH-4bp/Wts (2a4b) mice after a hyperimmunization protocol involving sheep red blood cells. Analysis of cell suspensions derived from the splenic tumors revealed a mixed lymphoid phenotype of the total cell population. Both the T-cell and B-cell populations remained after multiple passages of the tumors in syngeneic mice. A multiparameter approach combining immunologic and molecular genetic techniques was taken to determine the malignant cell population within these lymphomas. This analysis included depletion experiments utilizing histocompatible (B10.A X 21M)F1 mice to distinguish normal host-derived cells from the malignant tumor cells, fluorescence-activated cell sorting of the two cell populations followed by in vivo growth of the sorted cells, in vitro and in vivo cloning attempts, and molecular analysis of genomic DNA derived from the splenic tumors for clonal rearrangements of the immunoglobulin heavy-chain and T-cell receptor loci. Each of the three lymphomas was shown to be a malignant B-cell tumor that has an associated nonneoplastic T-cell component. The nature and functional significance of the numerous T-cells present in the B-cell lymphomas remain unknown and are being investigated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8874
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
80
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
351-60
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Mixed phenotype lymphomas of B10.H-2a-H-4bp/Wts mice: characterization of component cell populations.
pubmed:affiliation
Department of Microbiology and Immunology, University of North Carolina at Chapel Hill.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.