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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1988-4-22
|
| pubmed:databankReference | |
| pubmed:abstractText |
Transgenic mice were constructed with a functional T cell receptor beta gene. Transcription of the introduced gene is largely confined to T cells, but low levels of transcripts are also seen in B cells and in other tissues. Serological analyses show that most, if not all, of the T lymphocytes express the transgenic beta chain on the cell surface and lack beta chains encoded by endogenous beta genes. Molecular genetic analyses of uncloned and cloned T lymphocytes demonstrate that rearrangement of endogenous beta genes is incomplete. Partial D beta 1-J beta 1 rearrangements are found preferentially, while complete VDJ rearrangements are not seen. These findings show that expression of the transgene regulates the rearrangement of endogenous beta genes. Although the alpha beta T cell receptors of the transgenic mice are homogeneous with respect to the beta chain, they are fully functional, at least in a variety of allogeneic responses.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0092-8674
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
52
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
831-41
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3258191-Animals,
pubmed-meshheading:3258191-Base Sequence,
pubmed-meshheading:3258191-Cell Membrane,
pubmed-meshheading:3258191-Cells, Cultured,
pubmed-meshheading:3258191-Clone Cells,
pubmed-meshheading:3258191-Cytotoxicity, Immunologic,
pubmed-meshheading:3258191-Female,
pubmed-meshheading:3258191-Gene Expression Regulation,
pubmed-meshheading:3258191-Genes,
pubmed-meshheading:3258191-Male,
pubmed-meshheading:3258191-Mice,
pubmed-meshheading:3258191-Mice, Transgenic,
pubmed-meshheading:3258191-Molecular Sequence Data,
pubmed-meshheading:3258191-Nucleic Acid Hybridization,
pubmed-meshheading:3258191-Receptors, Antigen, T-Cell,
pubmed-meshheading:3258191-T-Lymphocytes,
pubmed-meshheading:3258191-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:3258191-Transcription, Genetic
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
In transgenic mice the introduced functional T cell receptor beta gene prevents expression of endogenous beta genes.
|
| pubmed:affiliation |
Basel Institute for Immunology, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|