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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1988-4-18
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pubmed:abstractText |
The intracellular free Ca2+ concentration ([Ca2+]i) of the bullfrog sympathetic ganglion cell was measured with fura-2 fluorescence under various conditions, and compared with changes in membrane potential recorded with an intracellular electrode. The [Ca2+]i was 109 nM on average under the resting condition and increased by raising the extracellular K+, stimulating repetitively the pre- or post-ganglionic nerve, or by applying acetylcholine or muscarine. Since all these procedures depolarized the cell membrane, most of the rise in [Ca2+]i could be the result of opening of voltage-dependent Ca2+ channels. However, Ca2+ entries through nicotinic acetylcholine receptor channels and the channel activated by the muscarinic acetylcholine receptor were also indicated by considering the threshold for the opening of voltage-dependent Ca2+ channels (for both entries) or a limited number of the cells showing the latter response.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Muscarine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cholinergic
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-8993
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
438
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
175-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3257890-Acetylcholine,
pubmed-meshheading:3257890-Adrenergic Fibers,
pubmed-meshheading:3257890-Animals,
pubmed-meshheading:3257890-Benzofurans,
pubmed-meshheading:3257890-Calcium,
pubmed-meshheading:3257890-Electric Stimulation,
pubmed-meshheading:3257890-Fura-2,
pubmed-meshheading:3257890-Membrane Potentials,
pubmed-meshheading:3257890-Muscarine,
pubmed-meshheading:3257890-Rana catesbeiana,
pubmed-meshheading:3257890-Receptors, Cholinergic
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pubmed:year |
1988
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pubmed:articleTitle |
Measurement of intracellular Ca2+ in the bullfrog sympathetic ganglion cells using fura-2 fluorescence.
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pubmed:affiliation |
Department of Physiology, Saga Medical School, Japan.
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pubmed:publicationType |
Journal Article,
In Vitro
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