Linked Life Data

3257582

Source:http://linkedlifedata.com/resource/pubmed/id/3257582

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-2-29
pubmed:abstractText
Evaluation of lymphokine production by individual activated T cells is necessary to characterize their growth requirements. We have studied interleukin 2 (IL-2) secretion by mature T lymphocytes using a high resolution assay system with the following characteristics: (a) a threshold of IL-2 detection 25 times lower than classic IL-2 titration; (b) the ability to discriminate between IL-2 and IL-4 activities; (c) absence of 'in situ' IL-2 absorption; (d) IL-2 production revealed at the single cell level. By this method an average of 10% of spleen cells, and 75% of L3T4+ cells were detected as producers in a concanavalin A (Con A)-dependent T cell activation system. Our results also suggest the complete restriction of IL-2 secretion to cells with this phenotype. Therefore, factors other than IL-2 must play a major role in Lyt 2+ mitogen-driven, helper-independent T cell proliferation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0300-9475
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-57
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Limiting dilution analysis of interleukin-2-producing mature T cells. Interleukin 2 secretion is an exclusive property of L3T4+ lymphocytes.
pubmed:affiliation
Unité INSERM U 25, Hôpital Necker, Paris, France.
pubmed:publicationType
Journal Article