Linked Life Data

3257511

Source:http://linkedlifedata.com/resource/pubmed/id/3257511

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-3-15
pubmed:abstractText
We here describe a form of 'noise' in the ELISA as commonly performed on antigen-coated microtiter trays that represents a major hindrance to the accurate enumeration of infrequent antibody-forming cell (AFC) precursors (AFCp) specific for epitopes on monomeric proteins. Supernatants from cultures of lipopolysaccharide-stimulated murine splenocytes, when split into aliquots and separately assayed, scored as positive in parallel on ELISA trays coated with unrelated proteins and on uncoated trays. Some properties of such coincident false positives (CFP) noted were: (1) optical density (OD) ranges for CFP and non-CFP overlapped; (2) different members of CFP triplets on differently coated assay trays usually had similar OD values; (3) CFP-generating culture supernatants did not contain unusually high immunoglobulin concentrations; and (4) numbers of CFP-forming supernatants increased with increasing input cells/culture consistent with causation by single AFCp present at an approximate mean frequency of 1 in 6600 CBA splenocytes. It is proposed that CFP are due to AFC clones that secrete antibody reactive with some epitope(s) present in the assay tray itself. Repertoire elements with such 'anti-plastic' characteristics are rarer than anti-keyhole limpet hemocyanin (KLH) AFCp, but at least as frequent as anti-bovine serum albumin (BSA) or anti-transferrin (TFN) AFCp.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
181-9
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Analysis of the B lymphocyte repertoire by polyclonal activation. Hindrance by clones yielding antibodies which bind promiscuously to plastic.
pubmed:affiliation
Walter and Eliza Hall Institute of Medical Research, Post Office Royal Melbourne Hospital, Victoria, Australia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't