Linked Life Data

3257425

Source:http://linkedlifedata.com/resource/pubmed/id/3257425

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-3-3
pubmed:abstractText
T lymphocytes on the epithelial surface of the lower respiratory tract are thought to represent a relatively compartmentalized population of T cells that exchanges slowly with the blood. Since the lung is chronically burdened with antigens, "resident" T cells likely have a history of past activation. To evaluate this concept, we analyzed resident lung T cells for VLA-1 expression, which is indicative of a history of past stimulation. Lung lavage and blood T cells were evaluated in 13 normal nonsmokers using the monoclonal antibodies Leu4 (pan T cells), Leu3 (helper/inducer T cells), Leu2 (suppressor/cytotoxic T cells), TS2/7 (alpha 1 subunit of VLA-1), and A-1A5 (beta subunit of VLA-1) using immunofluorescence and immunoprecipitation. In contrast to normal blood T cells which did not express VLA-1, lung T cells expressed the 210-kDa alpha 1 and 130-kDa beta subunits of the VLA-1 complex, the same as blood T cells activated in culture for 3 weeks. Two-color immunofluorescence with Leu4 and TS2/7 showed that 19 +/- 6% of the lung T cells were VLA-1+, suggesting that a significant proportion of T lymphocytes on the alveolar epithelial surface are in a separate compartment from the VLA-1 blood cells. In sarcoidosis, a disease characterized by exaggerated numbers of active Leu3+ T cells in the lower respiratory tract, increased numbers of lung Leu3+ T cells expressing VLA-1 were present on the epithelial surface of the lung (P less than 0.05 compared to normals). These observations are consistent with compartmentalized, chronically stimulated T lymphocytes on the alveolar epithelial surface that exchange with the systemic immune system very slowly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0090-1229
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
221-33
pubmed:dateRevised
2002-11-1
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
T lymphocytes compartmentalized on the epithelial surface of the lower respiratory tract express the very late activation antigen complex VLA-1.
pubmed:affiliation
Pulmonary Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.
pubmed:publicationType
Journal Article