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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1989-9-27
|
| pubmed:abstractText |
Mast cells isolated from the peritoneal fluid of Wistar rats were purified by centrifugation on Percoll gradient with a yield of 2x10(6) cells/ml. Cell morphology was well preserved as shown by light and electron microscopy. The mast cells capacity to bind histamine was assayed using either [3H]-histamine or histamine-ferritin conjugate as electron-opaque probe for electron microscopic examination. The [3H]-histamine binding performed at 4 degrees C in Ca2+-free phosphate-buffered saline pH 7.3 completed within 30 min was found to be specific, with an IC50 value of 0.72 +/- 0.23 nM. The data analyses by Scatchard and Hill's representations showed a KD of 0.60 +/- 0.24 nM and Bmax of 4.9 +/- 1.2 pM/10(6) cells suggesting that on mast cells the histamine receptors are restricted to the plasma membrane. According to Hill's analysis neither positive nor negative cooperativity (n = 1.06) appeared to be involved in the specific histamine-receptor binding. Competition experiments with 4-methylhistamine and SK&F 93479, revealed that mast cells express H2-histamine receptors. At electron microscopic level, the histamine-ferritin conjugate interstitially injected in the hamster cheek pouch was localized on the mast cell membrane.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0740-9451
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
469-88
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3255880-Animals,
pubmed-meshheading:3255880-Binding, Competitive,
pubmed-meshheading:3255880-Cells, Cultured,
pubmed-meshheading:3255880-Cheek,
pubmed-meshheading:3255880-Cricetinae,
pubmed-meshheading:3255880-Male,
pubmed-meshheading:3255880-Mast Cells,
pubmed-meshheading:3255880-Microscopy, Electron,
pubmed-meshheading:3255880-Rats,
pubmed-meshheading:3255880-Rats, Inbred Strains,
pubmed-meshheading:3255880-Receptors, Histamine,
pubmed-meshheading:3255880-Temperature
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Histamine receptor on mast cells.
|
| pubmed:affiliation |
Institute of Cellular Biology and Pathology, Bucharest, Romania.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|