Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1989-9-12
pubmed:abstractText
The glucocorticoid hormones methyl prednisolone and dexamethasone were shown to be cytostatic, but not cytotoxic, at high cell densities for early passage and continuous cell lines from human glioma at 0.25 microM and above, in the presence or absence of serum. In the absence of serum both steroids at 2.5 nM increased the saturation density close to the level reached in serum. Examination of the iodinated glycoproteins of the cell surface by gel electrophoresis did not reveal any consistent change. However, gel exclusion chromatography of protease digests of the cell surface and of material released into the medium showed an increase in incorporation of 3H-glucosamine in pronase digests after treatment with methyl prednisolone. Ion exchange chromatography showed that sulphated glycosaminoglycans, particularly heparan sulphate, increased and hyaluronic acid decreased in response to steroids, and there was increased retention of GAGs on the cell surface relative to the released fraction. It was concluded that glucocorticoid hormones modify the cell surface of human glioma cells and that this may contribute to enhanced cell intraction and lead to increased density limitation of cell proliferation.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0306-9443
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:3254724-Astrocytoma, pubmed-meshheading:3254724-Cell Division, pubmed-meshheading:3254724-Cell Line, pubmed-meshheading:3254724-Chromatography, Gel, pubmed-meshheading:3254724-Chromatography, Ion Exchange, pubmed-meshheading:3254724-Dexamethasone, pubmed-meshheading:3254724-Dose-Response Relationship, Drug, pubmed-meshheading:3254724-Drug Evaluation, Preclinical, pubmed-meshheading:3254724-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:3254724-Glioma, pubmed-meshheading:3254724-Glucocorticoids, pubmed-meshheading:3254724-Glycoproteins, pubmed-meshheading:3254724-Glycosaminoglycans, pubmed-meshheading:3254724-Humans, pubmed-meshheading:3254724-Membrane Proteins, pubmed-meshheading:3254724-Methylprednisolone, pubmed-meshheading:3254724-Surface Properties, pubmed-meshheading:3254724-Tumor Cells, Cultured
pubmed:year
1988
pubmed:articleTitle
Glucocorticoids and the cell surface of human glioma cells: relationship to cytostasis.
pubmed:affiliation
C.R.C. Department of Medical Oncology, University of Glasgow, UK.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't