Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1989-8-25
pubmed:abstractText
An enzyme-linked immuno sorbent assay has been set up for the quantitation of the elastin crosslink desmosine. With the assay desmosine could be detected in the range 0.01-10 ng (the amount is corresponding to 0.4-400 ng/ml) in standard solutions. One hundred percent crossreactivity with isodesmosine was found. The low titer rabbit antiserum used in this inhibition immunoassay was less suitable for the measurement of desmosine in urinary hydrolysates, because a number of unknown urinary substances interfered with the measurement. Interfering substances could not be removed completely with a column purification method. Future research is needed to determine the applicability of the higher titer anti-desmosine antiserum from a second rabbit.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0385-0005
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-63
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Experience with an enzyme-linked immuno sorbent assay for the quantitation of urinary desmosine.
pubmed:affiliation
Department of Public Health, School of Medicine, Tokai University, Kanagawa, Japan.
pubmed:publicationType
Journal Article