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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1989-5-26
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pubmed:abstractText |
A rapid, convenient assay for flavin-containing monooxygenase activity is described. The method is based on direct analysis of quenched incubation mixtures by reverse-phase liquid chromatography, and utilizes p-nitrophenyl-1,3-oxathiolane as the substrate. The synthesis of the substrate and the product are described. The usefulness of p-nitrophenyl-1,3-oxathiolane S-oxide formation as a measure of flavin-containing monooxygenase activity was demonstrated using highly purified and microsomal hog and rat liver flavin-containing monooxygenase. The assay is especially useful for determining stereoselectivity of flavin-containing monooxygenase activity in small amounts of crude tissue preparations.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Heterocyclic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrobenzenes,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/Thiophenes,
http://linkedlifedata.com/resource/pubmed/chemical/dimethylaniline monooxygenase...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0003-2697
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
274-80
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3245574-Animals,
pubmed-meshheading:3245574-Chromatography, High Pressure Liquid,
pubmed-meshheading:3245574-Heterocyclic Compounds,
pubmed-meshheading:3245574-Microsomes, Liver,
pubmed-meshheading:3245574-Nitrobenzenes,
pubmed-meshheading:3245574-Oxygenases,
pubmed-meshheading:3245574-Rats,
pubmed-meshheading:3245574-Swine,
pubmed-meshheading:3245574-Thiophenes
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pubmed:year |
1988
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pubmed:articleTitle |
A reverse-phase liquid chromatographic assay for flavin-containing monooxygenase activity.
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pubmed:affiliation |
Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco 94143.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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