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pubmed-article:3243228pubmed:abstractTextA method allowing a clear separation of the different variants of desialylated alpha 1-acid glycoprotein (orosomucoid) has been developed using isoelectric focusing in immobilized pH gradients, supplemented with 8 M urea and 2% v/v 2-mercaptoethanol. Immunoblotting with two antibody-steps afforded high sensitivity and permitted the detection of about 700 pg of alpha 1-acid glycoprotein in a 20 microL plasma sample diluted 1:28 672. A one year old bloodstrain, kept at room temperature, could easily be phenotyped.lld:pubmed
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pubmed-article:3243228pubmed:articleTitleIsoelectric focusing of alpha-1 acid glycoprotein (orosomucoid) in immobilized pH-gradients with 8M urea: detection of its desialylated variants using an alkaline phosphatase-linked secondary antibody system.lld:pubmed
pubmed-article:3243228pubmed:affiliationClinique psychiatrique universitaire de Lausanne Hôpital de Cery, Prilly-Lausanne, Switzerland.lld:pubmed
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