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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1989-6-5
|
| pubmed:abstractText |
A method allowing a clear separation of the different variants of desialylated alpha 1-acid glycoprotein (orosomucoid) has been developed using isoelectric focusing in immobilized pH gradients, supplemented with 8 M urea and 2% v/v 2-mercaptoethanol. Immunoblotting with two antibody-steps afforded high sensitivity and permitted the detection of about 700 pg of alpha 1-acid glycoprotein in a 20 microL plasma sample diluted 1:28 672. A one year old bloodstrain, kept at room temperature, could easily be phenotyped.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0173-0835
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
650-4
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:3243228-Alkaline Phosphatase,
pubmed-meshheading:3243228-Blood Stains,
pubmed-meshheading:3243228-Genetic Variation,
pubmed-meshheading:3243228-Humans,
pubmed-meshheading:3243228-Hydrogen-Ion Concentration,
pubmed-meshheading:3243228-Immunoblotting,
pubmed-meshheading:3243228-Isoelectric Focusing,
pubmed-meshheading:3243228-Neuraminidase,
pubmed-meshheading:3243228-Orosomucoid,
pubmed-meshheading:3243228-Phenotype,
pubmed-meshheading:3243228-Urea
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Isoelectric focusing of alpha-1 acid glycoprotein (orosomucoid) in immobilized pH-gradients with 8M urea: detection of its desialylated variants using an alkaline phosphatase-linked secondary antibody system.
|
| pubmed:affiliation |
Clinique psychiatrique universitaire de Lausanne Hôpital de Cery, Prilly-Lausanne, Switzerland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|