Linked Life Data

3241663

Source:http://linkedlifedata.com/resource/pubmed/id/3241663

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1989-5-16
pubmed:abstractText
We have examined the distribution of specific binding of the 5-HT3 receptor ligand [3H]GR65630 to rat brain using quantitative autoradiography. Brain tissue was frozen and thin sections (20 microns) cut. Slide-mounted tissue sections were incubated with [3H]GR65630 (0.2 nM) in the presence or absence of metoclopramide (30 microM, to define non-specific binding). After drying, sections were apposed to photographic film for 3 months. Films were quantified for radioactivity using image analysis. The highest level of specific binding was found in the area postrema (34.0 fmol/mg tissue). Specific binding was high in intermediate layers of the cortex, particularly the entorhinal, temporal and pyriform cortex (5.2-7.0 fmol/mg tissue). Amongst sub-cortical areas, binding was evident in the olfactory lobes, olfactory tubercle, hippocampus and basolateral amygdala (1.3-3.3 fmol/mg tissue). Specific binding was below measurable levels in other sub-cortical areas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
156-60
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
The distribution of specific binding of the 5-HT3 receptor ligand [3H]GR65630 in rat brain using quantitative autoradiography.
pubmed:affiliation
Department of Neuropharmacology, Glaxo Group Research Ltd., Ware, U.K.
pubmed:publicationType
Journal Article