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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1977-6-30
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pubmed:abstractText |
A lipoprotein mutant of E. coli K-12 has been characterized. The mutant lipoprotein was found to differ from the wild-type lipoprotein in the following respects: (i) it is present in an appreciable amount in the soluble fraction (275,000 X g supernatant); (ii) it lacks the covalently-linked diglyceride; (iii) it contains an unmodified cysteine which can be carboxymethylated in vitro; (iv) it undergoes dimerization and the dimer can be converted into monomeric form by reduction with 2-mercaptoethanol; (v) both the monomeric form and especially the dimeric form of the mutant lipoprotein migrate more slowly than the corresponding forms of wild-type lipoprotein in sodium dodecyl sulfate/urea polyacrylamide gel electrophoresis; and (vi) the mutant lipoprotein is not assembled into the murein sacculi, and this results in a greatly reduced amount of bound-form lipoprotein in the mutant. These data strongly suggest that the mutation has affected the primary structure of lipoprotein, in such a way that it is not modified normally, leading to the production of a structurally-altered lipoprotein deficient in covalently-linked lipid as well as a defective assembly of the altered lipoprotein into the rigid layer of the cell envelope.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4101989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4245367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4261992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4565677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4575979,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4579427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4580564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4592401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4899922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-4905995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-5506260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-58861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-767331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-770420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-783146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/323849-816772
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1388-92
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1977
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pubmed:articleTitle |
Biochemical characterization of a mutant lipoprotein of Escherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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