Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1989-4-26
|
| pubmed:abstractText |
Two novel beta-oxidation products of peptido leukotrienes, 16-carboxy-17,18,19,20-tetranor-14,15-dihydro-N-acetyl LTE4 and 18-carboxy-19,20-dinor-N-acetyl LTE4, were prepared by total synthesis and used to identify previously unknown polar rat biliary metabolites. When [3H] LTC4 and synthetic N-acetyl-LTE4 were administered intravenously to anesthetized inbred male rats, extraction of the bile and subsequent reverse-phase HPLC fractionation allowed the isolation of two novel metabolites of N-acetyl-LTE4. Comparison of U.V. spectra and coelution experiments revealed that these metabolites correspond to the above-mentioned synthetic beta-oxidation products. This was further confirmed by the coelution of the corresponding methyl esters. Oxidative ozonolysis of the metabolically produced 16-carboxy-17,18,19,20-tetranor-14,15-dihydro-N-acetyl LTE4 (major metabolite) confirmed the absence of the 14,15-unsaturation. The presence of these metabolites indicates that peptide leukotrienes undergo N-acetylation followed by omega and subsequent beta-oxidation in the anesthetized rat.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0090-6980
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
291-302
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading | |
| pubmed:year |
1988
|
| pubmed:articleTitle |
Synthesis of beta-oxidation products as potential leukotriene metabolites and their detection in bile of anesthetized rat.
|
| pubmed:affiliation |
Department of Medicinal Chemistry, Merck Frosst Canada Inc., Pointe Claire-Dorval, Québec.
|
| pubmed:publicationType |
Journal Article
|