Linked Life Data

3226625

Source:http://linkedlifedata.com/resource/pubmed/id/3226625

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
1989-3-29
pubmed:abstractText
The soluble proteins of bovine chromaffin granules were subjected to 2D-electrophoresis followed by immunoblotting with an antiserum against the pituitary peptide 7B2. One immunoreactive spot was visualized at a position corresponding to a molecular weight of 24,000 and to a pI of 5.2. Using peroxidase-antiperoxidase (PAP) pre-embedding immunocytochemical technique for electron microscopy, 7B2 has been localized within secretory granules with diameters of approximately 115 and 190 nm in noradrenergic and adrenergic cells respectively. These data establish that in chromaffin granules 7B2 represents a minor component of the acidic proteins which include the chromogranins A and B, secretogranin II and the enkephalin-containing peptides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0304-3940
pubmed:author
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Two-dimensional immunoblotting analysis and immunocytochemical localization of the secretory polypeptide 7B2 in adrenal medulla.
pubmed:affiliation
J.A. De Sève Laboratories of Molecular and Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, Que., Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't