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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1989-4-6
|
| pubmed:abstractText |
We monitored intracellular pH (pHi) in isolated perfused S3 segments of the rabbit proximal tubule, and studied the effect of acetate (Ac-) transport on pHi. pHi was calculated from the absorbance spectrum of 4',5'-dimethyl-5-(and 6) carboxyfluorescein trapped intracellularly. All solutions were nominally HCO3(-)-free. Removal of 10 mM Ac- from bath and lumen caused pHi to rapidly rise by approximately 0.2, and then to decline more slowly to a value approximately 0.35 below the initial one. Removal of only luminal Ac- caused pHi changes very similar to those resulting from bilateral removal of Ac-. When Ac- was removed from bath only, pHi rose rapidly at first, and then continued to rise more slowly. Readdition of Ac- to bath caused pHi to rapidly fall to a value slightly higher than the one prevailing before the removal of Ac- from the bath. In experiments in which Ac- was first removed from both bath and lumen, readdition of 10 mM Ac- to only lumen caused a rapid but small acidification, followed by a slower alkalinization that brought the pHi near the value that prevailed before the bilateral removal of Ac-. The alkalinizing effects caused by the readdition of 10 or 0.5 mM Ac- were indistinguishable. When Ac- was returned to only lumen in the absence of luminal Na+, there was a small and rapid pHi decrease, but no pHi recovery. Removal of Na+ from bath did not affect the pHi transients caused by the addition of Ac- to lumen. In experiments in which Ac- was first removed bilaterally, readdition of Ac- to only bath caused a large and sustained drop in pHi, whereas the subsequent removal of Ac- from the bath caused a slight alkalinization. These pHi changes caused by readdition or removal of Ac- from baths were unaffected by the absence of external Na+. We conclude that there is a Na+/Ac- cotransporter at the luminal membrane, and pathways for acetic acid transport at both luminal and basolateral membranes. The net effect of Ac- transport on pHi is to alkalinize the cell as a result of the luminal entry of Na+/Ac-, which is followed by the luminal and basolateral exit of acetic acid.
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| pubmed:grant | |
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-14829580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-291013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-3225554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-3440860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-3515013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-36128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-3822761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-3888071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-3888090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-4093954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-6159793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-6318566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-6331176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-6614155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-6742135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-6842580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-6849384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-6854625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-7155794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-7237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-7452725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3225555-956381
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0022-1295
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
92
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
395-412
|
| pubmed:dateRevised |
2009-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:3225555-Acetates,
pubmed-meshheading:3225555-Animals,
pubmed-meshheading:3225555-Biological Transport,
pubmed-meshheading:3225555-Female,
pubmed-meshheading:3225555-Hydrogen-Ion Concentration,
pubmed-meshheading:3225555-Kidney Tubules, Proximal,
pubmed-meshheading:3225555-Kinetics,
pubmed-meshheading:3225555-Perfusion,
pubmed-meshheading:3225555-Phosphates,
pubmed-meshheading:3225555-Rabbits,
pubmed-meshheading:3225555-Sodium
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Acetate transport in the S3 segment of the rabbit proximal tubule and its effect on intracellular pH.
|
| pubmed:affiliation |
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut 06510.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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