rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1989-4-4
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pubmed:databankReference |
|
pubmed:abstractText |
The cloning and sequencing of an Aspergillus niger gene encoding a secreted form of phosphate-repressible acid phosphatase by complementation of a pacA (phosphate-repressible acid phosphatase) mutant of Aspergillus nidulans is described. The gene contains two introns, 201 and 265 nt in length, and codes for a 1.6-kb transcript. Both phosphate concentration and pH of the growth medium affect the level of expression of the gene in A. niger. Similar regulation is observed in A. nidulans transformants. A putative signal peptide, resembling known signal sequences of yeast, is identified.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0378-1119
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pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
30
|
pubmed:volume |
71
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
339-48
|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3224828-Acid Phosphatase,
pubmed-meshheading:3224828-Amino Acid Sequence,
pubmed-meshheading:3224828-Aspergillus niger,
pubmed-meshheading:3224828-Base Sequence,
pubmed-meshheading:3224828-Cloning, Molecular,
pubmed-meshheading:3224828-DNA, Recombinant,
pubmed-meshheading:3224828-Enzyme Repression,
pubmed-meshheading:3224828-Genes, Fungal,
pubmed-meshheading:3224828-Hydrogen-Ion Concentration,
pubmed-meshheading:3224828-Introns,
pubmed-meshheading:3224828-Molecular Sequence Data,
pubmed-meshheading:3224828-Mutation,
pubmed-meshheading:3224828-Phosphates,
pubmed-meshheading:3224828-Restriction Mapping,
pubmed-meshheading:3224828-Transcription, Genetic,
pubmed-meshheading:3224828-Transformation, Genetic
|
pubmed:year |
1988
|
pubmed:articleTitle |
A phosphate-repressible acid phosphatase gene from Aspergillus niger: its cloning, sequencing and transcriptional analysis.
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pubmed:affiliation |
Allelix Inc., Mississauga, Ont., Canada.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|