Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1989-3-27
pubmed:abstractText
Labelled membrane proteins from freshly isolated or over-night cultured rat hepatocytes were compared using 2-D gel electrophoresis. The membrane protein patterns changed rapidly after culturing, some changes being apparent after 5 hours in culture. These changes included the disappearance of a number of membrane proteins and the apparent altered glycosylation of others. Stability of hepatocyte membrane proteins in culture did not appear to be related to the stage of hepatocyte differentiation. A recognition that rapid changes in the membrane protein composition may occur after culturing is important for those wishing to use cultured cells to study membrane-associated functions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0309-1651
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1011-25
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
2-D gel comparison of membrane proteins from freshly isolated and cultured fetal and adult hepatocytes.
pubmed:affiliation
Pathology Department, University of Melbourne, Parkville, Victoria, Australia.
pubmed:publicationType
Journal Article, Comparative Study