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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
|
pubmed:dateCreated |
1989-3-27
|
pubmed:abstractText |
Labelled membrane proteins from freshly isolated or over-night cultured rat hepatocytes were compared using 2-D gel electrophoresis. The membrane protein patterns changed rapidly after culturing, some changes being apparent after 5 hours in culture. These changes included the disappearance of a number of membrane proteins and the apparent altered glycosylation of others. Stability of hepatocyte membrane proteins in culture did not appear to be related to the stage of hepatocyte differentiation. A recognition that rapid changes in the membrane protein composition may occur after culturing is important for those wishing to use cultured cells to study membrane-associated functions.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Dec
|
pubmed:issn |
0309-1651
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
12
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1011-25
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3224375-Aging,
pubmed-meshheading:3224375-Animals,
pubmed-meshheading:3224375-Cell Differentiation,
pubmed-meshheading:3224375-Cells, Cultured,
pubmed-meshheading:3224375-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:3224375-Fetus,
pubmed-meshheading:3224375-Iodine Radioisotopes,
pubmed-meshheading:3224375-Liver,
pubmed-meshheading:3224375-Membrane Fluidity,
pubmed-meshheading:3224375-Membrane Proteins,
pubmed-meshheading:3224375-Rats
|
pubmed:year |
1988
|
pubmed:articleTitle |
2-D gel comparison of membrane proteins from freshly isolated and cultured fetal and adult hepatocytes.
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pubmed:affiliation |
Pathology Department, University of Melbourne, Parkville, Victoria, Australia.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|