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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1989-3-17
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pubmed:abstractText |
A lectin that displays specificity for the core region of asparagine-linked oligosaccharides (Man3GlcNAc2-Asn) was isolated from human serum and liver by affinity chromatography on mannan-Sepharose. The designation 'core-specific lectin' (CSL) is used to indicate its specificity. Selective elution of human CSL from mannan-Sepharose was accomplished with 50 mM-mannose. Two additional proteins that displayed Ca2+-dependent binding to mannan-Sepharose were eluted by mannose 6-phosphate or beta-glycerophosphate but not by mannose. The latter proteins were identified as C-reactive protein and serum amyloid protein. Human CSL isolated from liver was indistinguishable from serum CSL in its physicochemical properties, immunological properties and specificity. The N-terminal sequence of human CSL is homologous to that reported for 'mannan-binding protein C' (MBP-C) [Drickamer, Dordal & Reynolds (1986) J. Biol. Chem. 261, 6878-6887]. The amino acid composition of human CSL is similar to that of rat MBP-C, including the presence of hydroxyproline and hydroxylysine residues. Collagen-like sequences with hydroxylated proline and lysine residues appear to be present in human CSL as well as in rat CSL. The collagen-like regions of human and rat CSL may play a role in assembly of CSL subunits into complexes consisting of nine subunits that display Ca2+-dependent carbohydrate-binding activity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-23915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-2820982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-3003043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-3009480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-3112140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-3584121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-3611062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-3741882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-3818649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-6266278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-6643429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-666781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-6706987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-6774714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-6847200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-6847642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-7012150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-7037761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-7061511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-7086148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-7263640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3223912-7305977
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
61-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3223912-Amino Acid Sequence,
pubmed-meshheading:3223912-Amino Acids,
pubmed-meshheading:3223912-Carbohydrate Metabolism,
pubmed-meshheading:3223912-Carbohydrates,
pubmed-meshheading:3223912-Chromatography, Gel,
pubmed-meshheading:3223912-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3223912-Humans,
pubmed-meshheading:3223912-Lectins,
pubmed-meshheading:3223912-Liver,
pubmed-meshheading:3223912-Mannose-Binding Lectin,
pubmed-meshheading:3223912-Molecular Sequence Data,
pubmed-meshheading:3223912-Proteins
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pubmed:year |
1988
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pubmed:articleTitle |
Purification and characterization of the core-specific lectin from human serum and liver.
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pubmed:affiliation |
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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