Linked Life Data

3221173

Source:http://linkedlifedata.com/resource/pubmed/id/3221173

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1989-3-23
pubmed:abstractText
Monoclonal antibodies against human thyroglobulin were produced by the hybridoma technique. Using three non-crossreactive monoclonal antibodies, an IRMA system was developed, with a polyclonal rabbit antibody fixed on microtiter plates as first extracting antibody. The monoclonal antibodies were used as second antibody, while anti-mouse IgG-biotin from goat and [125I]streptavidin served as the indicator system. The source of the monoclonal antibodies was diluted culture medium without purification. Sensitivities of 3-4 micrograms/l were obtained with all 3 monoclonal antibodies; interassay variation was about 5%. This test system will be used for further immunological characterization of circulating thyroglobulin in different thyroid diseases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0340-076X
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
435-9
pubmed:dateRevised
2007-7-24
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Development of immunoradiometric assays for human thyroglobulin using monoclonal antibodies and the biotin/avidin system.
pubmed:affiliation
Medizinische Klinik, Universität Heidelberg, Mannheim.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't