|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1989-2-13
|
|
pubmed:abstractText |
The distribution of calmodulin-binding polypeptides in various rat liver subcellular fractions was investigated. Plasma-membrane, endosome, Golgi and lysosome fractions were prepared by established procedures. The calmodulin-binding polypeptides present in the subcellular fractions were identified by using an overlay technique after transfer from gels to nitrocellulose sheets. Distinctive populations of calmodulin-binding polypeptides were present in all the fractions examined except lysosomes. A major 115 kDa calmodulin-binding polypeptide of pI 4.3 was located to the endosome subfractions, and it emerges as a candidate endosome-specific protein. Partitioning of endosome fractions between aqueous and Triton X-114 phases indicated that the calmodulin-binding polypeptide was hydrophobic. Major calmodulin-binding polypeptides of 140 and 240 kDa and minor polypeptides of 40-60 kDa were present in plasma membranes. The distribution of calmodulin in the various endosome and plasma-membrane fractions was also analysed, and the results indicated that the amounts were high compared with those in the cytosol.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-125584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-211139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2415534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2859513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2867759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2867760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2867761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2878931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2888775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2890445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2937787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2983664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-2983716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3007150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3011784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3031085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3257572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3304132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3312195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3477810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3512582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3606592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3611090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-3954776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-4028107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-4850204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6086671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6091623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6128074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6169732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6207183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6233611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6239867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6243188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6257680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6296143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6435623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6699090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6758951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6832135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3214436-6954520
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
255
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
999-1005
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:3214436-Animals,
pubmed-meshheading:3214436-Calmodulin-Binding Proteins,
pubmed-meshheading:3214436-Cell Membrane,
pubmed-meshheading:3214436-Cytosol,
pubmed-meshheading:3214436-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3214436-Endocytosis,
pubmed-meshheading:3214436-Intracellular Membranes,
pubmed-meshheading:3214436-Liver,
pubmed-meshheading:3214436-Peptides,
pubmed-meshheading:3214436-Rats,
pubmed-meshheading:3214436-Subcellular Fractions
|
|
pubmed:year |
1988
|
|
pubmed:articleTitle |
A 115 kDa calmodulin-binding protein is located in rat liver endosome fractions.
|
|
pubmed:affiliation |
Laboratory of Protein Structure, National Institute for Medical Research, London, U.K.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
