Linked Life Data

3208769

Source:http://linkedlifedata.com/resource/pubmed/id/3208769

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1989-2-14
pubmed:abstractText
The amount of mRNA for the low-density-lipoprotein (LDL) receptor in cultured human fibroblasts was estimated by hybridization of the poly(A)-rich RNA fraction with a DNA probe, using the recovery of beta-actin mRNA to correct for losses. During incubation of the cells with lipoprotein-deficient serum (LPDS) both the LDL-receptor mRNA content and the rate of receptor protein synthesis increased fourfold during the first 16 h and then fell by approximately 50% during the next 24 h. The content of beta-actin mRNA fell by a similar amount, so that the ratio of receptor/beta-actin mRNAs rose and then remained constant. The fall in beta-actin mRNA content during incubation with LPDS was not prevented by the addition of cholesterol to the medium. In cells from a homozygous familial hypercholesterolaemic (FH) subject that bound 20% of the normal amount of LDL, the content of LDL-receptor mRNA and the changes during incubation with LPDS or free sterols were similar to normal. Cells from a familial hypercholesterolaemic subject that produced no immunodetectable receptor protein produced a small amount of receptor mRNA of apparently normal size which responded in the same way as in normal cells to LPDS and free sterols.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
178
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
555-61
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Low-density-lipoprotein-receptor mRNA content of fibroblasts from normal and familial hypercholesterolaemic subjects.
pubmed:affiliation
Medical Research Council Lipoprotein Team, Hammersmith Hospital, London, England.
pubmed:publicationType
Journal Article