Linked Life Data

3207804

Source:http://linkedlifedata.com/resource/pubmed/id/3207804

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1989-2-23
pubmed:abstractText
Glucose uptake was measured by a noninvasive fluorescence technique on a total of 165 morula- and blastocyst-stage murine embryos in two different culture media. Eighty-four embryos were tested in M2 medium, and the remaining 81 embryos were tested in M16. Embryos assayed in M2 took up significantly less glucose over the 4-h assay period than did embryos assayed in M16. The lower uptake of glucose by embryos in M2 corresponded with a decrease in the quality of embryos cultured overnight in M2 as judged by morphological criteria. Embryos that were judged to be degenerate or had gross abnormalities took up significantly less glucose than did normal embryos. Glucose uptake in both populations of embryos covered a wide range of values and was normally distributed. A significant effect between mothers was noted in glucose uptake for embryos assayed in both M16 and M2 media. The possible uses of noninvasive measures of glucose uptake as a test of embryo viability or for optimizing culture conditions are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
779-86
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Noninvasive measurement of glucose uptake by two populations of murine embryos.
pubmed:affiliation
Department of Physiology and Biophysics, Harvard Medical School, Boston, Massachusetts 02115.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.