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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1989-2-6
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pubmed:abstractText |
Cell proliferation kinetics in a spontaneous mouse fibrosarcoma (FSaII) growing in C3H mice has been studied by in vivo pulse labeling of cells synthesizing DNA with bromodeoxyuridine (BrdUrd). A monoclonal antibody to BrdUrd and flow cytometry were used to quantify these cells. Labeling indices (LI) were measured before and after radiation. Unirradiated 10-mm tumors had a mean LI of 17.5%. After a single dose of 20 Gy there was depression of LI after 1 day followed by a rapid increase to greater than control values after 5 days. Analysis performed after five fractions showed that LI was dependent on the dose per fraction and interval between fractions. After 5 and 7 Gy/fraction LI remained similar to control values during daily fractionation but was significantly depressed after twice daily fractionation. With doses greater than 10 Gy/fraction there was marked depression of LI using both fractionation schedules. These changes in LI correlated well with changes in tumor volume after radiation. Tumors were also biopsied after 5 fractions of a 20-fraction course to see if LI would predict for tumor control. LIs of greater than or equal to 10% were associated with lack of tumor control at 90 days while all controlled tumors had a significant depression of LI. Changes in LI after radiation were a reasonable indication of the amount of repopulation occurring and might be useful in selecting patients for altered fractionation schedules.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0033-7587
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
116
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
453-61
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3205909-Animals,
pubmed-meshheading:3205909-Bromodeoxyuridine,
pubmed-meshheading:3205909-Cell Division,
pubmed-meshheading:3205909-Female,
pubmed-meshheading:3205909-Fibrosarcoma,
pubmed-meshheading:3205909-Flow Cytometry,
pubmed-meshheading:3205909-Male,
pubmed-meshheading:3205909-Mice,
pubmed-meshheading:3205909-Mice, Inbred C3H
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pubmed:year |
1988
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pubmed:articleTitle |
Changes in bromodeoxyuridine labeling index during radiation treatment of an experimental tumor.
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pubmed:affiliation |
Edwin L. Steele Laboratory of Radiation Biology, Massachusetts General Hospital, Harvard Medical School, Boston 02114.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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