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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1989-2-6
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pubmed:abstractText |
The 5S gene-specific transcription factor TFIIIA forms an exceptionally stable complex with the internal promoter of the 5S RNA gene. Approximately 1 to 5% of TFIIIA-DNA or deoxyoligonucleotide complexes are stable to harsh denaturation conditions and can be resolved by electrophoresis in the presence of SDS. These complexes are resistant to acidic conditions (0.1 N HCl, 55 degrees C, 2h) suggesting that the interaction may be through a covalent bond. Complex formation does not result in DNA strand scission and studies of the chemical sensitivity of the complex suggest that the TFIIIA-DNA linkage may be through a phosphoramidate bond. Covalent complexes are formed with both the noncoding (RNA-like) and coding strands of the internal control region. The predominant sites of TFIIIA-DNA adducts have been mapped to the 3' end of the 5S gene internal control region, the region previously shown to exhibit essential guanine and phosphate contacts with TFIIIA.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-2421409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-2427927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-2445754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-2830454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-2837652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-284338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-2992360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3013416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3096579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3319186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3515197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3560227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3683556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3791417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3796733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3935797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3967290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-3972795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-4007166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-4040853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-4910303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6153931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6159099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6196359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6233272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6246487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6248230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6263489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6269756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6275366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6297765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6327057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6356356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6369252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6540147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-6722885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-7001457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3205743-7060135
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 5S,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Dodecyl Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIIIA,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11267-84
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3205743-Animals,
pubmed-meshheading:3205743-DNA-Binding Proteins,
pubmed-meshheading:3205743-Gene Conversion,
pubmed-meshheading:3205743-Macromolecular Substances,
pubmed-meshheading:3205743-Nucleic Acid Denaturation,
pubmed-meshheading:3205743-Oligonucleotide Probes,
pubmed-meshheading:3205743-Promoter Regions, Genetic,
pubmed-meshheading:3205743-RNA, Ribosomal,
pubmed-meshheading:3205743-RNA, Ribosomal, 5S,
pubmed-meshheading:3205743-Sodium Dodecyl Sulfate,
pubmed-meshheading:3205743-Transcription Factor TFIIIA,
pubmed-meshheading:3205743-Transcription Factors,
pubmed-meshheading:3205743-Xenopus laevis
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pubmed:year |
1988
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pubmed:articleTitle |
Xenopus transcription factor IIIA forms a complex of covalent character with 5S DNA.
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pubmed:affiliation |
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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