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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
23
|
| pubmed:dateCreated |
1989-1-24
|
| pubmed:abstractText |
Chromogranin B 420-493 (GAWK)-like immunoreactivity (chromogranin B (420-493)-LI) was determined by radioimmunoassay using two different rabbit antisera, one raised against chromogranin B (420-436) (GAWK 1-17) (Ab420-436) and the other against chromogranin B 439-457 (GAWK 20-38) (Ab439-457), in bovine and human tissues. Chromogranin B (420-493)-LI was present in the bovine adrenal medulla chromaffin granules as well as in the anterior pituitary gland and was released from the cultured bovine chromaffin cells by stimulation with high K+ or nicotine. Chromogranin B (420-493)-LI present in the bovine tissues was detected using Ab420-436 but was not detected using Ab439-457. In the human tissues, chromogranin B (420-493)-LI was detected using Ab420-436 as well as Ab439-457. This suggests that the amino acid sequence of this region (chromogranin B 439-457) is different between human and bovine. On the gel permeation chromatography, chromogranin B (420-493)-LI was eluted at the void volume in the bovine adrenal medulla and at an apparent molecular weight of 4000 in the anterior pituitary gland. On the reverse-phase high-performance liquid chromatography, multiple peaks of chromogranin B (420-493)-LI was detected in the bovine adrenal medulla while one component of chromogranin B (420-493)-LI was found in the anterior pituitary gland. These results suggest that chromogranin B is processed into small fragments of chromogranin B (420-493)-LIs and that this processing is tissue-specific.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CHGB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chromogranin B,
http://linkedlifedata.com/resource/pubmed/chemical/Chromogranins,
http://linkedlifedata.com/resource/pubmed/chemical/GAWK,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotine,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0024-3205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1945-52
|
| pubmed:dateRevised |
2007-4-17
|
| pubmed:meshHeading |
pubmed-meshheading:3200116-Adrenal Medulla,
pubmed-meshheading:3200116-Animals,
pubmed-meshheading:3200116-Cattle,
pubmed-meshheading:3200116-Cells, Cultured,
pubmed-meshheading:3200116-Chromaffin Granules,
pubmed-meshheading:3200116-Chromatography, Gel,
pubmed-meshheading:3200116-Chromatography, High Pressure Liquid,
pubmed-meshheading:3200116-Chromogranin B,
pubmed-meshheading:3200116-Chromogranins,
pubmed-meshheading:3200116-Humans,
pubmed-meshheading:3200116-Immunoassay,
pubmed-meshheading:3200116-Molecular Weight,
pubmed-meshheading:3200116-Nerve Tissue Proteins,
pubmed-meshheading:3200116-Nicotine,
pubmed-meshheading:3200116-Peptide Fragments,
pubmed-meshheading:3200116-Pituitary Gland, Anterior,
pubmed-meshheading:3200116-Potassium,
pubmed-meshheading:3200116-Tissue Distribution
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Different processing of chromogranin B into GAWK-immunoreactive fragments in the bovine adrenal medulla and pituitary gland.
|
| pubmed:affiliation |
Department of Biochemistry, National Kyushu Cancer Center, Fukuoka, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|