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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1989-1-19
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pubmed:abstractText |
The complete 897-amino-acid sequence of chicken skeletal muscle alpha-actinin and the 856-amino-acid sequence (97% of the entire sequence) of chicken fibroblast alpha-actinin have been determined by cloning and sequencing the cDNAs. Genomic Southern analysis with the cDNA sequences shows that skeletal and fibroblast alpha-actinins are encoded by separate single-copy genes. RNA blot analyzes show that the skeletal alpha-actinin gene is expressed in the pectoralis muscle and that the fibroblast gene is expressed in the gizzard smooth muscle as well as in the fibroblast. The deduced skeletal alpha-actinin molecule has a calculated Mr of 104 x 10(3), and each alpha-actinin can be divided into three domains: (1) the NH2-terminal highly conserved actin-binding domain, which shows similarity to the product of the Duchenne's muscular dystrophy locus; (2) the middle rod-shaped dimer-forming domain, which contains the spectrin-type repeat units; and (3) the COOH-terminal two EF-hand consensus regions. Comparison of the skeletal alpha-actinin sequence with the fibroblast and smooth muscle alpha-actinin sequences demonstrated that the EF-hand structure was conserved in all of these alpha-actinin sequences, despite the reported variability of the Ca2+ sensitivities of the actin-gelation by various alpha-actinin isoforms.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
649-55
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:3197725-Actinin,
pubmed-meshheading:3197725-Amino Acid Sequence,
pubmed-meshheading:3197725-Animals,
pubmed-meshheading:3197725-Base Sequence,
pubmed-meshheading:3197725-Cells, Cultured,
pubmed-meshheading:3197725-Chick Embryo,
pubmed-meshheading:3197725-Chickens,
pubmed-meshheading:3197725-DNA,
pubmed-meshheading:3197725-Fibroblasts,
pubmed-meshheading:3197725-Molecular Sequence Data,
pubmed-meshheading:3197725-Muscles,
pubmed-meshheading:3197725-Restriction Mapping,
pubmed-meshheading:3197725-Skin
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pubmed:year |
1988
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pubmed:articleTitle |
Primary structure of chicken skeletal muscle and fibroblast alpha-actinins deduced from cDNA sequences.
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pubmed:affiliation |
Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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