Linked Life Data

3197637

Source:http://linkedlifedata.com/resource/pubmed/id/3197637

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1989-1-17
pubmed:abstractText
In this study we have investigated a possible functional role of the plasma pattern of GH in regulation of insulin-like growth factor I (IGF-I) mRNA in liver, skeletal muscle, and rib growth plate of the rat. Hypophysectomized male rats given T4 and glucocorticoid replacement therapy were equipped with indwelling jugular venous cannulae attached via swivels to a multichannel pumping system programmed to deliver human GH in a continuous or pulsatile (one pulse per 3 h) pattern for 5 days. At the end of the experiment, skeletal muscle, rib growth plates, and liver from intact and hypophysectomized rats were homogenized, and total nucleic acid was prepared. IGF-I mRNA was quantitated by solution hybridization assay using a RNA probe radiolabeled with [35S]UTP. Pulsatile treatment with GH in a dose of 1.5 U/kg.day induced a 3- to 5-fold increase in the levels of IGF-I mRNA in skeletal muscle and rib growth plates. In contrast, continuous infusion with GH was much less effective in these tissues. In the liver both continuous and pulsatile GH infusion significantly elevated the amount of IGF-I mRNA, and there was no significant difference between these two treatments. In the tissues studied similar results were obtained with a higher dose of GH (3.0 U/kg.day). Pulsatile GH treatment stimulated longitudinal bone growth more effectively than continuous GH treatment, confirming earlier studies. It is concluded that pulsatile GH treatment is more effective than continuous GH infusion in increasing IGF-I mRNA levels in rib growth plate and skeletal muscle, i.e. two major target organs for the anabolic effects of GH.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
123
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2605-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Pulsatile intravenous growth hormone (GH) infusion to hypophysectomized rats increases insulin-like growth factor I messenger ribonucleic acid in skeletal tissues more effectively than continuous GH infusion.
pubmed:affiliation
Department of Physiology, University of Göteborg, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't