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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
23
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pubmed:dateCreated |
1988-12-30
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pubmed:abstractText |
The role of nuclear protooncogenes during erythroid cell differentiation was examined by transfecting exogenous c-fos and c-myb genes into mouse erythroleukemia cells, which can be induced to differentiate either with erythropoietin (Epo) or dimethyl sulfoxide. Expression of exogenous c-myb or c-fos oncogene completely inhibited Epo-induced erythroid differentiation but only partially inhibited dimethyl sulfoxide-induced differentiation. Normally Epo-induced differentiation leads to a drastic decline of c-myb mRNA levels and an increase of c-myc transcripts in the early stage of differentiation. Cells expressing exogenous c-fos gene, however, maintained high levels of c-myb mRNA after Epo treatment. This high level of c-myb transcripts was found to be due to block of transcription shutoff (or transcriptional activation) rather than to mRNA stabilization. It is concluded that the down-regulation of endogenous c-myb gene expression is a prerequisite for commitment of Epo-induced erythroid differentiation and that expression of c-myb gene may be indirectly regulated by c-fos gene product. We also concluded that early down-regulation of c-myc gene expression is not essential for erythroid differentiation and that gene regulation of chemically induced erythroid differentiation may differ from that of Epo-induced differentiation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194397-18467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194397-2430281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194397-2998780,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3194397-3085953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194397-3099293,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3194397-3119222,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3194397-7133118
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8900-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3194397-Animals,
pubmed-meshheading:3194397-Cell Differentiation,
pubmed-meshheading:3194397-Cell Line,
pubmed-meshheading:3194397-DNA, Neoplasm,
pubmed-meshheading:3194397-DNA Probes,
pubmed-meshheading:3194397-Erythropoietin,
pubmed-meshheading:3194397-Gene Expression Regulation,
pubmed-meshheading:3194397-Half-Life,
pubmed-meshheading:3194397-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:3194397-Leukemia, Experimental,
pubmed-meshheading:3194397-Mice,
pubmed-meshheading:3194397-Proto-Oncogenes,
pubmed-meshheading:3194397-RNA, Messenger,
pubmed-meshheading:3194397-Transcription, Genetic,
pubmed-meshheading:3194397-Transfection
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pubmed:year |
1988
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pubmed:articleTitle |
Down-regulation of c-myb gene expression is a prerequisite for erythropoietin-induced erythroid differentiation.
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pubmed:affiliation |
Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki, Japan.
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pubmed:publicationType |
Journal Article
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