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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1989-1-5
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pubmed:abstractText |
Virtually all models for recombination between homologous DNA sequences invoke a branched intermediate known as a Holliday structure. The terminal steps of recombination are postulated to involve a specific cleavage through the four-way junction of a Holliday structure, in a process known as resolution. We have constructed a synthetic Holliday structure in which the position of the junction of the DNA duplexes can branch migrate through approximately 185 bp. Using this structure, we have found that a component of a cytoplasmic extract of Hela cells is capable of cleaving the central junction of the substrate in a manner consistent with resolution. The activity requires a divalent cation but does not require an exogenous energy source. This is the first reported resolution activity from a mammalian source.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-1063393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-2823121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-2843646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-2935376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3036850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3277721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3303029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3304134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3375068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3396544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3485474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3491821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-386340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3901001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-3903750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-6092381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-6092961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-6099255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-6288255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-6297377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194199-6597761
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10249-66
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
Resolution of synthetic Holliday structures by an extract of human cells.
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pubmed:affiliation |
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06510.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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