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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1989-1-5
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pubmed:abstractText |
The chloroplast gene rps12 for ribosomal protein S12 in a liverwort, Marchantia polymorpha, is split into three exons by two introns, one of which (intron 1) is discontinuous. Exon 1 of rps12 for the N-terminal portion of the S12 protein is far from exons 2 and 3 for the C-terminal portion on the opposite DNA strand. S1-nuclease protection analysis and Northern hybridization with RNA isolated from the liverwort chloroplasts showed that: (i) the exons 1 and 2-3 of the rps12 gene with the neighboring genes were transcribed separately, (ii) the trans-splicing of intron 1 occurred after the processing of two primary transcripts to two pre-mRNAs, and (iii) there was no particular order for the splicing of intron 1 (trans) and intron 2 (cis) in the rps12 gene. We propose a bimolecular interaction model for trans-splicing by assuming that intermolecular base pairings between two pre-mRNAs result in the formation of the structure typical of group II introns except for disruption in the loop III region. This structure could be constructed in intron 1 of tobacco rps12 gene.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-11894905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-16453785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-2430332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3042383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3280139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3297351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3422433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3502722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3594568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3791410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3848347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3848348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3945556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-3960739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-6357781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-6989816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3194192-922889
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10025-36
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:3194192-Base Sequence,
pubmed-meshheading:3194192-Blotting, Northern,
pubmed-meshheading:3194192-Chloroplasts,
pubmed-meshheading:3194192-Genes,
pubmed-meshheading:3194192-Introns,
pubmed-meshheading:3194192-Molecular Sequence Data,
pubmed-meshheading:3194192-Nucleic Acid Conformation,
pubmed-meshheading:3194192-Nucleic Acid Hybridization,
pubmed-meshheading:3194192-Plants,
pubmed-meshheading:3194192-RNA, Messenger,
pubmed-meshheading:3194192-RNA Precursors,
pubmed-meshheading:3194192-RNA Splicing,
pubmed-meshheading:3194192-Ribosomal Proteins,
pubmed-meshheading:3194192-Transcription, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
A nicked group II intron and trans-splicing in liverwort, Marchantia polymorpha, chloroplasts.
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pubmed:affiliation |
Research Center for Cell and Tissue Culture, Kyoto University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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