Linked Life Data

3192154

Source:http://linkedlifedata.com/resource/pubmed/id/3192154

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1989-1-6
pubmed:abstractText
A procedure to measure chloramphenicol acetyl transferase (CAT) activity by reverse-phase high-performance liquid chromatography is described. The antibiotic as well as the acetylated derivatives are well resolved on a Superspher RP-18 column using equal parts of acetonitrile and 10 mM sodium acetate (ph 5.0) as a solvent. Under these conditions, less than 100 pmol of each derivative can be easily detected within 10 minutes, and no radioactive chloramphenicol is needed. The present procedure has been used to measure the activity of the enzyme in extracts of chicken fibroblast transfected with the recombinant plasmid pSV2-cat containing the CAT gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0735-0651
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Assay of chloramphenicol acetyl transferase by high-performance liquid chromatography.
pubmed:affiliation
Instituto de Bioquimica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't