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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1977-3-15
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pubmed:abstractText |
Sheep erythrocytes sensitized by incubation with the heat-labile enterotoxin (LT) of Escherichia coli are hemolyzed in the presence of anti-LT antiserum and complement. The Microtiter (Cooke Laboratory Products) technique was used to titrate anti-LT antibody in serum by this immune hemolysis reaction. Immune hemolysis was inhibited by preexposure of the anti-LT antiserum to soluble LT before addition of the LT-sensitized sheep cells. E. coli mini-extract preparations were obtained by the polymyxin release technique and assayed for LT by the lysis inhibition test (LIT) and by the adrenal cell assay. All 75 adrenal cell-positive E. coli isolates were positive in the LIT assay. Eight of 318 adrenal cell-negative isolates tested were positive in the LIT assay, possibly indicating the presence of biologically inactive toxin.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-1091855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-1097914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-1101807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-163964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-16557689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-16558081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-176284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4356461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4553083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4560518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4577415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4581006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4584048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4584049,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4593468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4595989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-4610064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-5460905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/319105-772435
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
100-5
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pubmed:dateRevised |
2010-9-2
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pubmed:meshHeading |
pubmed-meshheading:319105-Animals,
pubmed-meshheading:319105-Enterotoxins,
pubmed-meshheading:319105-Erythrocytes,
pubmed-meshheading:319105-Escherichia coli,
pubmed-meshheading:319105-Hemolysis,
pubmed-meshheading:319105-Hot Temperature,
pubmed-meshheading:319105-Humans,
pubmed-meshheading:319105-Immune Sera,
pubmed-meshheading:319105-Immunologic Techniques,
pubmed-meshheading:319105-Serotyping,
pubmed-meshheading:319105-Sheep
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pubmed:year |
1977
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pubmed:articleTitle |
Inhibition of immune hemolysis: serological assay for the heat-labile enterotoxin of Excherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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