Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7A
|
| pubmed:dateCreated |
1988-12-5
|
| pubmed:abstractText |
Antigenicity study of N2-[(N-acetylmuramoyl)-L-alanyl-D-isoglutaminyl]-N6-stearoyl-L-lysi++ + ne (MDP-Lys(L18), muroctasin) was carried out in mice, guinea pigs and rabbits with passive cutaneous anaphylaxis (PCA), systemic anaphylaxis (SA), Arthus and delayed skin reaction and enzyme-linked immunosorbent assay (ELISA). Mice were sensitized intraperitoneally, twice at 3-week intervals, with MDP-Lys(L18) (40, 400 or 4000 micrograms/kg) or MDP-Lys(L18)-ovalbumin (OA) conjugate (500 micrograms/kg) in alumina gel. No IgE antibodies to MDP-Lys(L18) were detected in the sera obtained from the sensitized mice by 24-h PCA in rat. Guinea pigs were sensitized subcutaneously with MDP-Lys(L18) (4, 40 or 400 micrograms/kg) or MDP-Lys(L18)-OA (2 mg/kg) emulsified in Freund's complete and incomplete adjuvant, 3 times at 2-week intervals. No SA was observed in the sensitized animals after the intravenous injection of MDP-Lys(L18) (1 mg/kg). Rabbits were sensitized subcutaneously with MDP-Lys(L18) (2 or 20 micrograms/kg) or MDP-Lys(L18)-OA (2 mg/kg) emulsified in Freund's incomplete adjuvant, 3 times at 2-week intervals. Neither Arthus nor delayed skin reaction was observed in the sensitized animals after the intradermal injection of MDP-Lys(L18) (2 micrograms/site). Although antibodies to MDP-Lys(L18) were not detected in MDP-Lys(L18) sensitized animals, the antibodies were detected in two out of 9 guinea pigs and all of the rabbits in the MDP-Lys(L18)-OA sensitized groups by 4-h PCA after the intravenous injection of MDP-Lys(L18) (1 mg/kg). These results suggest that MDP-Lys(L18) may possess antigenic potential by PCA reaction in guinea pigs.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0004-4172
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1034-7
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3190797-Acetylmuramyl-Alanyl-Isoglutamine,
pubmed-meshheading:3190797-Adjuvants, Immunologic,
pubmed-meshheading:3190797-Anaphylaxis,
pubmed-meshheading:3190797-Animals,
pubmed-meshheading:3190797-Antibody Formation,
pubmed-meshheading:3190797-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3190797-Female,
pubmed-meshheading:3190797-Guinea Pigs,
pubmed-meshheading:3190797-Hypersensitivity, Delayed,
pubmed-meshheading:3190797-Mice,
pubmed-meshheading:3190797-Mice, Inbred BALB C,
pubmed-meshheading:3190797-Passive Cutaneous Anaphylaxis,
pubmed-meshheading:3190797-Rabbits
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Antigenicity study of muroctasin.
|
| pubmed:affiliation |
Research Institute, Daiichi Seiyaku Co., Ltd., Tokyo, Japan.
|
| pubmed:publicationType |
Journal Article
|