Linked Life Data

3189884

Source:http://linkedlifedata.com/resource/pubmed/id/3189884

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-12-15
pubmed:abstractText
Within the adult mammalian ovary, angiogenesis is associated with development of the corpus luteum (CL). In this study, developing luteal tissue was examined to determine whether its vascularization involves endothelial cell replication and to what extent this proliferation contributes to forming new capillaries. Five rats each at 12, 24, 36, 48, 60, 72, 84, and 96 hr of gestation were given a subcutaneous injection of tritiated thymidine (specific activity 5 Ci/mM; 1 uCi/g body weight). One hour later they were anesthetised with sodium pentobarbitone, and the left ovary was processed for light microscopy. Sections were cut through each ovary until three newly formed CL were recognized; a 1-micron section was taken from the maximum diameter of each CL and processed for autoradiographic demonstration of thymidine labeling in endothelial cell nuclei. The same sections were also examined with stereological techniques to quantitate growth of the vascular compartment. The results show that 36.1 +/- 5.7% of endothelial cells of invading capillary sprouts divide within 12 hr of ovulation; at 24 hr, 29.0 +/- 2.8% are dividing. Within 12 hr after ovulation, blood vessels occupied 5.9 +/- 1.4% of the peripheral space of the ruptured follicle but only 1.6 +/- 0.5% in the center. However, by 36 hr these respective values were 9.3 +/- 1.6% and 8.4 +/- 1.9%. A further peak in endothelial-cell replication (31.2 +/- 5.4%), early on the 3rd day of gestation, corresponded to the very extensive anastomoses within the capillary bed established between this time (13.6% vascularity) and late on the 4th day (about 23%).(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0003-276X
pubmed:author
pubmed:issnType
Print
pubmed:volume
222
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18-25
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Angiogenesis in the developing corpus luteum of pregnant rats: a stereologic and autoradiographic study.
pubmed:affiliation
Department of Anatomy and Human Biology, University of Western Australia, Nedlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't