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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0013682,
umls-concept:C0028606,
umls-concept:C0071499,
umls-concept:C0332464,
umls-concept:C0348011,
umls-concept:C0439599,
umls-concept:C0443288,
umls-concept:C0486805,
umls-concept:C0812409,
umls-concept:C1444662,
umls-concept:C1514721,
umls-concept:C1705822,
umls-concept:C1706746,
umls-concept:C2699488
|
| pubmed:issue |
2
|
| pubmed:dateCreated |
1988-12-14
|
| pubmed:abstractText |
A vertical electrophoresis procedure utilizing a discontinuous polyacrylamide gradient agarose gel was developed to resolve DNA fragments ranging in size from over 50 kb to less than 300 bp in length. The gel consisted of a polyacrylamide plug at the base of the gel followed by a gradient of agarose ranging from 0.3 to 0.9%. Restriction fragments migrated shorter distances than in a comparable polyacrylamide-0.3% agarose gel, and small fragments were retained. Southern transfer of DNA fragments from the gradient gel onto nitrocellulose was more efficient than transfer of fragments using a nongradient gel.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
173
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
285-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1988
|
| pubmed:articleTitle |
Discontinuous polyacrylamide gradient agarose gels resolve a wide range of restriction fragments and optimize the efficiency of nucleic acid transfer.
|
| pubmed:affiliation |
Covalent Technology Corporation, Ann Arbor, Michigan 48106.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|