Linked Life Data

3188393

Source:http://linkedlifedata.com/resource/pubmed/id/3188393

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-12-14
pubmed:abstractText
The cauliflower mosaic virus (CaMV) 35 S RNA is a full-length transcript of the viral genome. It encodes the genes VII and I-V, arranged in tandem along the RNA, preceded by a long leader region (600 bases) containing many short open reading frames. We have examined the effects of the leader and the first gene (gene VII) on downstream gene I translation in vitro and in an in vivo transient expression system (carrot protoplasts). RNAs from constructs containing the intact leader, and from various deletion constructs, were translated in a rabbit reticulocyte system. Gene I was translated efficiently only when the long leader region and the upstream gene VII were deleted. Translational fusions of gene VII or I to the firefly luciferase reporter gene were also constructed, and a similar series of leader sequence deletion mutants were examined in vivo and in vitro. The 600-base leader region was found to repress translation of gene VII 8- to 30-fold as compared to the truncated gene lacking the leader region. Gene I expression as compared to that of gene VII was reduced an additional 7- to 20-fold by the presence of the upstream leader region including gene VII. This represented an overall reduction in gene I expression of greater than 100-fold as compared to expression in the absence of any leader sequence. The reduced translation of gene I in the context of the 35 S RNA leader region was not due to the action of the gene VII protein product but may result from efficient blocking of scanning 40 S ribosomes by translation of upstream open reading frames.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
167
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-35
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Cauliflower mosaic virus 35 S RNA leader region inhibits translation of downstream genes.
pubmed:affiliation
Department of Biology, University of California, San Diego, La Jolla 92093.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.