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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1988-12-20
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pubmed:abstractText |
In addition to members of the Orthomyxoviridae and Paramyxoviridae, several coronaviruses have been shown to possess receptor-destroying activities. Purified bovine coronavirus (BCV) preparations have an esterase activity which inactivates O-acetylsialic acid-containing receptors on erythrocytes. Diisopropyl fluorophosphate (DFP) completely inhibits this receptor-destroying activity of BCV, suggesting that the viral enzyme is a serine esterase. Treatment of purified BCV with [3H]DFP and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the proteins revealed that the E3 protein was specifically phosphorylated. This finding suggests that the esterase/receptor-destroying activity of BCV is associated with the E3 protein. Furthermore, treatment of BCV with DFP dramatically reduced its infectivity in a plaque assay. It is assumed that the esterase activity of BCV is required in an early step of virus replication, possibly during virus entry or uncoating.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-13426178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-14321178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-2411539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-2825419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-2999443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3016304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3304138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3356980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3380803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3414182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3589663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3660588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3681266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3700379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-3984492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-4118317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-4131955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-4138483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-4357516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-4361457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-4472498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-6199888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-6256942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-6258295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-6362367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-7086972,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3184275-841855
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4686-90
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:3184275-Acetylesterase,
pubmed-meshheading:3184275-Animals,
pubmed-meshheading:3184275-Cattle,
pubmed-meshheading:3184275-Cell Line,
pubmed-meshheading:3184275-Coronaviridae,
pubmed-meshheading:3184275-Glycoproteins,
pubmed-meshheading:3184275-Hemagglutination, Viral,
pubmed-meshheading:3184275-Hemagglutination Tests,
pubmed-meshheading:3184275-Isoflurophate,
pubmed-meshheading:3184275-Receptors, Virus,
pubmed-meshheading:3184275-Viral Plaque Assay,
pubmed-meshheading:3184275-Viral Proteins,
pubmed-meshheading:3184275-Virus Replication
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pubmed:year |
1988
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pubmed:articleTitle |
The E3 protein of bovine coronavirus is a receptor-destroying enzyme with acetylesterase activity.
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pubmed:affiliation |
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029-6574.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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