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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1988-11-21
|
| pubmed:abstractText |
The extent and rate of disassembly of microtubules induced by the addition of high concentrations of magnesium and calcium have been measured. At 25 degrees C, the rate constant for microtubule disassembly increases more than ten-fold on increasing [Mg2+] from 4.0 to 20 mM. The process is even more sensitive to [Ca2+], showing similar enhancement on increasing [Ca2+] from 0.5 to 8 mM. Electron microscopy indicates that the disassembly is an end-dependent process. Complete microtubule disassembly occurs at concentrations in excess of 10 and 2 mM for Mg2+ and Ca2+, respectively; this suggests the importance of binding to weak sites for both ions. The sensitivity to ionic composition explains the wide variations in the published values for k-, under varying conditions. The results indicate the potential range of microtubule disassembly rates which may be encountered under different conditions in vitro and in vivo. The highest values of k- (ca. 3000 s-1) would imply microtubule shortening rates in excess of 100 um per minute.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
30
|
| pubmed:volume |
155
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1464-70
|
| pubmed:dateRevised |
2010-8-25
|
| pubmed:meshHeading | |
| pubmed:year |
1988
|
| pubmed:articleTitle |
Fast disassembly of microtubules induced by Mg2+ or Ca2+.
|
| pubmed:affiliation |
Institute of Biophysics, Faculty of Medicine, University of Belgrade.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|