Linked Life Data

3178234

Source:http://linkedlifedata.com/resource/pubmed/id/3178234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-11-17
pubmed:abstractText
The levels of dolichyl phosphate and 2,3-dehydrodolichyl diphosphate synthase were determined in seminiferous tubules of prepuberal rats to assess any changes occurring during early stages of spermatogenesis. Dolichyl phosphate increased in concentration two- to threefold from Day 10 to Day 23 after birth. A method was optimized to measure dehydrodolichyl diphosphate synthesis from delta 3-[14C]isopentenyl diphosphate and t,t-farnesyl diphosphate in homogenates of seminiferous tubules. Both dehydrodolichyl mono- and diphosphates were observed as products of the in vitro assay. The specific activity of tubular synthase increased twofold between Day 7 and Day 23 and decreased similarly between Day 23 and Day 60. Since there was a parallel increase in the concentration of tubular dolichyl phosphate and dehydrodolichyl diphosphate synthase activity during early stages of spermatogenesis, it is proposed that the level of dolichyl phosphate may be controlled at least in part by the regulation of de novo dehydrodolichyl diphosphate biosynthesis. The synthase was also solubilized from tubular membranes with deoxycholate and partially purified by chromatography.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:volume
266
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
98-110
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Changes in dehydrodolichyl diphosphate synthase during spermatogenesis in the rat.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.