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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1988-11-22
|
| pubmed:abstractText |
Metabolically competent isolated cerebral cortical nerve terminals were used to determine the effects of triethyllead (TEL) and triethyltin (TET) on cytosolic free calcium ([Ca2+]c), on plasma and mitochondrial membrane potentials, and on oxidative metabolism. In the presence of physiological concentrations of extracellular ions, 20 microM TEL and 20 microM TET increase [Ca2+]c from 185 nM to 390 and 340 nM, respectively. A simultaneous depolarization of plasma membrane potential (delta psi p) by only 3-4 mV occurs, a drop which is insufficient to open the voltage-sensitive Ca2+ channels. In contrast, an instant and substantial depolarization of mitochondrial membrane potential (delta psi m) upon addition of TEL and TET is evident, as monitored with safranine O fluorescence. At the same concentration, TEL and TET stimulate basal respiration of synaptosomes by 45%, induce oxidation of endogenous NAD(P)H, and reduce the terminal ATP/ADP ratio by 45%. Thus, TEL and TET inhibit ATP production of intrasynaptosomal mitochondria by a mechanism consistent with uncoupling of oxidative phosphorylation. This bioenergetic effect by TEL and TET can be prevented by omitting external chloride, and a concomitant reduction of the increase in [Ca2+]c by about 60% is observed. Uncoupling of mitochondrial ATP synthesis from oxidation by TEL and TET, [corrected] a process that is dependent on external chloride, is the main mechanism by which they [corrected] increase [Ca2+]c.
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| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonyl Cyanide m-Chlorophenyl...,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/NADP,
http://linkedlifedata.com/resource/pubmed/chemical/Organometallic Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Trialkyltin Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Triethyltin Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/triethyllead,
http://linkedlifedata.com/resource/pubmed/chemical/triethyltin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
51
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1617-25
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3171593-Adenosine Diphosphate,
pubmed-meshheading:3171593-Adenosine Triphosphate,
pubmed-meshheading:3171593-Animals,
pubmed-meshheading:3171593-Calcium,
pubmed-meshheading:3171593-Carbonyl Cyanide m-Chlorophenyl Hydrazone,
pubmed-meshheading:3171593-Cerebral Cortex,
pubmed-meshheading:3171593-Chlorides,
pubmed-meshheading:3171593-Cytosol,
pubmed-meshheading:3171593-Guinea Pigs,
pubmed-meshheading:3171593-Membrane Potentials,
pubmed-meshheading:3171593-Mitochondria,
pubmed-meshheading:3171593-NADP,
pubmed-meshheading:3171593-Organometallic Compounds,
pubmed-meshheading:3171593-Oxidative Phosphorylation,
pubmed-meshheading:3171593-Spectrometry, Fluorescence,
pubmed-meshheading:3171593-Synaptosomes,
pubmed-meshheading:3171593-Trialkyltin Compounds,
pubmed-meshheading:3171593-Triethyltin Compounds
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Chloride-dependent uncoupling of oxidative phosphorylation by triethyllead and triethyltin increases cytosolic free calcium in guinea pig cerebral cortical synaptosomes.
|
| pubmed:affiliation |
Department of Clinical Neurophysiology, University Central Hospital, Kuopio, Finland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|