Linked Life Data

3166443

Source:http://linkedlifedata.com/resource/pubmed/id/3166443

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1988-10-24
pubmed:abstractText
Plants containing the left T-DNA (TL) of Agrobacterium rhizogenes show a variety of developmental abnormalities that include severely wrinkled leaves, loss of apical dominance, reduced geotropism of roots, reduced internode distances, and floral hyperstyly. The TL-DNA also affects the morphology of tumor tissue at the site of inoculation on Kalanchoe diagremontiana leaves. Single mutations at four loci of the TL-DNA (rolA, rolB, rolC, and rolD) are known to affect tumor morphology on K. diagremontiana leaves. We regenerated plants from tissues transformed with TL-DNA containing mutations in each of the rol loci in order to determine which of the rol loci, if any, control the abnormal plant phenotype. Only plants regenerated after infection with bacteria containing a mutation in rolA locus showed loss of the wrinkled leaf phenotype. The rolA locus was cloned into the plant transformation vector pGA472 and introduced alone into plants. Transgenic plants containing rolA displayed the abnormal phenotype. These results indicate that rolA is the primary determinant of the severely wrinkled phenotype of Ri plasmid transgenic plants. Other rol loci may influence the degree of developmental abnormalities.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
688-97
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
rolA locus of the Ri plasmid directs developmental abnormalities in transgenic tobacco plants.
pubmed:affiliation
Department of Biochemistry, University of Washington, Seattle 98195.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't