Linked Life Data

3163937

Source:http://linkedlifedata.com/resource/pubmed/id/3163937

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
13
pubmed:dateCreated
1988-7-20
pubmed:abstractText
Experiments were performed with K562 erythroleukemia cells to further characterize the observation that hemin protects hemopoietic cells from the cytotoxic effects of anthracycline drugs. The present studies demonstrate that this protective effect of hemin applies only to anthracyclines and not to other classes of antineoplastic agents. Hemin interferes with the cellular accumulation of various anthracyclines, as measured by cytofluorography, and prevents binding of anthracyclines to isolated cell nuclei. Exposure of K562 cells to hemin retards the anthracycline-induced arrest of cells at the G2-M interphase of the cell cycle and permits cells to undergo continuing division as demonstrated by clonal growth in plasma clot cultures. Furthermore, hemin decreases the ability of anthracyclines to unwind simian virus 40 supercoiled DNA in vitro. The protective effect of hemin fails to occur if cells have been preincubated with this agent for 72 h before they are exposed to Adriamycin in the absence of hemin. The findings suggest that hemin prevents anthracycline-induced cytotoxicity by acting at several levels. These effects may be mediated by direct interactions of hemin with DNA and perhaps other cellular constituents or by molecular complex formation between hemin and anthracyclines at intracellular sites.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3566-70
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Analysis of hemin-induced protection of human hemopoietic cells from the cytotoxic effects of anthracyclines.
pubmed:affiliation
Charles A. Dana Research Institute, Department of Medicine, Beth Israel Hospital-Harvard Medical School, Boston, Massachusetts 02215.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't