|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1988-4-18
|
|
pubmed:abstractText |
Mutant CHO cell strains dependent upon cytidine for growth and survival were isolated by a direct selection protocol. The mutants obtained are deficient in CTP synthetase activity (less than 2% residual activity) and have low reversion frequencies (less than 10(-7)). Cytidine deprivation of these stains leads to rapid depletion of intracellular CTP pools, but not dCTP pools, and a surprisingly rapid loss of cell viability. These properties should make the cytidine auxotrophs useful for a number of biochemical and genetic studies.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0740-7750
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
14
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
149-54
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:3162335-Animals,
pubmed-meshheading:3162335-Carbon-Nitrogen Ligases,
pubmed-meshheading:3162335-Cell Line,
pubmed-meshheading:3162335-Cell Survival,
pubmed-meshheading:3162335-Cricetinae,
pubmed-meshheading:3162335-Cricetulus,
pubmed-meshheading:3162335-Culture Media,
pubmed-meshheading:3162335-Cytidine,
pubmed-meshheading:3162335-Ligases,
pubmed-meshheading:3162335-Mutation,
pubmed-meshheading:3162335-Selection, Genetic
|
|
pubmed:year |
1988
|
|
pubmed:articleTitle |
Direct selection of Chinese hamster ovary strains deficient in CTP synthetase activity.
|
|
pubmed:affiliation |
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Potters Bar, Herts., U.K.
|
|
pubmed:publicationType |
Journal Article
|
